cdk8 specific antibody  (Bethyl)

Journal: Chemical Science

Article Title: Controlled in-cell activation of RNA therapeutics using bond-cleaving bio-orthogonal chemistry † †Electronic supplementary information (ESI) available. See DOI: 10.1039/c7sc01380a Click here for additional data file.

doi: 10.1039/c7sc01380a

Figure Lengend Snippet: Western blotting analysis of CDK8 protein, MTT analysis and bright field microscopy measurements in MDA-MB-231 cells. (a) Western blotting analysis of CDK8 levels. Immobilization of siRNA on NP surface shuts down the activity of siRNA (lanes 2 vs. 1) whereas addition of the tetrazine 2 activates the siRNA resulting in a remarkable decrease in the expression of CDK8 (lanes 3 vs. 1). β-Actin was used as a loading control. (b) Relative levels of cell proliferation measured by MTT assay; activated siRNA slows down proliferation of MDA-MB-231 cells due to knockdown of the CDK8 gene. (c) Brightfield image illustrating decreased population of MDA-MB-231 cells after silencing of CDK8 gene with the activation of prodrug using tetrazine.

Article Snippet: A rabbit polyclonal antibody specific for CDK8, rabbit monoclonal antibody to GFP, rabbit monoclonal antibody for β-actin and horseradish peroxidase-conjugated secondary antibodies, used for chemiluminescence, were obtained from Cell Signaling Technology, (Danvers, MA, USA).

Techniques: Western Blot, Microscopy, Activity Assay, Expressing, Control, MTT Assay, Knockdown, Activation Assay